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Journal: Redox Report : Communications in Free Radical Research
Article Title: Elucidating the protective role of quercetin against lipopolysaccharide-induced necroptosis in broiler thymus: insights from Nrf2/PERK signaling based on network pharmacology and experimental validation
doi: 10.1080/13510002.2026.2663621
Figure Lengend Snippet: QUE attenuated necroptosis through Nrf2/PERK under LPS exposure. (A and B) IF results for RIPK3 and MLKL in thymus tissues ( n = 20 broilers per group). (C and D) The detection for mRNA and protein levels of necroptosis factors (RIPK1, RIPK3, Caspase8, MLKL, and P -MLKL) in thymus tissues ( n = 20 broilers per group). (E and F) IF results for necroptosis marker factors (RIPK3 and MLKL) in MSB-1 cells and quantitative analysis ( n = 3 independent cell culture experiments). (G and I) The transcription and protein levels of necroptosis factors, including RIPK1, RIPK3, Caspase8, MLKL, and P -MLKL, in MSB-1 cells ( n = 3 independent cell culture experiments). All the data are displayed as the means ± SD ( n = 3). The statistically significant differences are not denoted with the same letters, such as ‘a, b, c, and d’.
Article Snippet: Following dewaxing, the slices were subjected to antigen retrieval and blocked with 3% BSA at room temperature for 30 min. Then, the slices were incubated with PERK (GB11507-100, 1:200, Rabbit pAb, Servicebio, Wuhan), MLKL (GB155699-100, 1:5000, Rabbit pAb, Servicebio, Wuhan), and
Techniques: Marker, Cell Culture